Valine_Leucine
New member
Hey people,
I want to run a Fmoc deprotection of my peptide. The point is that I have an azide group in the side chain of the last aminoacid that I attached. Usually I run it with 20% piperidine in DMF but I am afraid about a possible side-reaction between the azido group and the free amino group after deprotection. I mean, which influence could have piperidine after the Fmoc deprotection in a possible reaction between azide and amino group (piperidine is a nucleophilic base...)
what about using DBU (non nucleophilic base)...?
Any suggestion or anyone of you have experienced with Fmoc deprotection having an azide group near...?
Thanks!
I want to run a Fmoc deprotection of my peptide. The point is that I have an azide group in the side chain of the last aminoacid that I attached. Usually I run it with 20% piperidine in DMF but I am afraid about a possible side-reaction between the azido group and the free amino group after deprotection. I mean, which influence could have piperidine after the Fmoc deprotection in a possible reaction between azide and amino group (piperidine is a nucleophilic base...)
what about using DBU (non nucleophilic base)...?
Any suggestion or anyone of you have experienced with Fmoc deprotection having an azide group near...?
Thanks!
