Best way to sterilize gear?

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g1234

Shut up and squat !
Below are several methods i collected from the web (most from Animal's website). What do you guys think? Which method would you use?

Method1: Filtering and BA
Filter the gear with a .2 micron filter and add 3-5% BA to the gear

Method2: Microwave Sterilization
This is the easiest method to use and fastest.

step 1 - YOU MUST TAKE THE METAL SEAL OFF THE TOP OF THE VIAL!

step 2 - Nothing should be covering the top of the vial. Take the seal and the
stopper off the top of the vial to where the oil is totally exposed. You can
do this with a pair of pliers or some instrument that can pry the lid off. The
seal will pry off pretty easily. I use a tool that is on my pocket knife. It
resembles a can opener.

step 3 - Place the vial in the microwave. Make sure there is nothing covering
the opening of the vial. Put the microwave on 20 seconds and hit start. After
it is through let sit for about 5 minutes and then repeat 2 more times.

step 4 - After the 3rd time immediately take the vial out of the microwave.
You may need mittens because it WILL be hot. At this point you should preload
all your syringes while the liquid is still hot. This is my preferred method
because it keeps everything sterile. Make sure to use 2 needles per syringe. 1
should be used to draw up the fluid. The other should be a clean one that will
be injected in your ass. The top of the second one should NOT BE REMOVED until
the time of injection. This ensures total sterility. If you do not want to
preload your syringes then youw ill need to put the rubber stopper back on the
syringe. Before you do that though plop it in the microwave for about 20
seconds on a paper towl or plate. Immediately take the stopper out and put it
on the vial using the napkin to pick it up with. This is a pain in the ass
though when the time comes to draw up the fluid. Not only do you expose the
oil once again when you take the seal off but you risk spilling the oil all
over the place. It is just easier to preload all the syringes when the oil is
still hot and sterile. Then you dont have to mess with it at a later date and
you can just inject

NOTE: I will be developing oil products soon. I will be using these same
techniques to sterilize the oils I will be selling. The vials will be
professionally sealed and will be ready to withdraw the fluid without first
sterilizing.

Method3: Boil
This takes some time cause you have to bring the water to a boil and then put
the vial in there. If you do not want to expose the oil because you will not
be preloading then I would use this method or the bake method.

step 1 - Stick a needle from a syringe through the rubber top of the vial so
that it is sticking through it by about a half an inch. The purpose of this is
to allow the BA to evaporate out of the vial. If it does not then the vial
WILL EXPLODE.

step 2 - Place the vial in a small pot. Fill the water level up to about a
half an inch below the top of the vial. Turn on the stovetop to about medium
high. You can even do high. (I do) Wait till the water starts boiling before
you start counting down the minutes. Let the vial sit in the water for 15
minutes then remove. (the vial may move around in the water as the bubbles
will push it around but it will not fall over)

step 3 - Sit the vial on a paper towl or other surface that can soak of any
water. Take the needle out of the top of the vial before it cools. If you let
it cool the needle will most likely get stuck there. It is actually smart to
leave it in there though. This way you can just put the syringe on needle and
draw up the fluid as you go. But if you do this make sure to put another
syringe on there when you are through with filling the other one. If you dont
you are leaving a small hole open to let bacteria get in. That is it for the
boil method. You can preload the syringes if you like after you take the vial
out. Beware though. It is a pain in the ass to attempt to preload the syringes
with the top on there and the liquid is still hot cause you will have to turn
it upside down to get the last bit of liquid in the syringes. That vial is
very hot!

Method 4: Bake Method
Bakind is pretty much the same procedure as the boil method. Just place the
vial in a pan and put a needle in the top. Bake 15 minutes on 250 degrees.
Wait about 10-15 min and repeat 2 more times.


I hope this helps some of you guys that may be weary about ordering IP gear
and other vet products. All oils CAN be heat sterilized. If you take the
correct procedures you will have IMO human grade products after you heat
sterilize the product. Besides....test testosterone propionate is test testosterone propionate after all. It doesnt
matter if a pharmaceutical company produces it or IP. As long as the dosages
are correct it works the same way in your body. The only difference is human
grade is sterile shipped to you and IP is not always sterile. It is just smart
to heat sterilize it. For the money you cannot beat private lab made gear, and
if proper sterilization processes are use it is the same thing as human grade.


IMPORTANT!
If your oil is shipped to you in a plastic container of some sort you cannot
obviously put this in boiling water or the oven. You can however put this in
the microwave for 20 seconds. If it is in a plastic container you must let the
product totally cool though before reheating it 2 more times in the microwave
or you risk the plastic vial melting.
 
F50 .2 micron filter is a good choice for that? i've seen some people use .45 - .5

Whats your reason for saying the other methods are wrong?
 
0.2 µm will filter cleaner than 0.45 µm
generally 0.2 µm is the largest pore size accepted to produce a sterile filtrate

methods 2, 3 and 4 revolve around the basis of heat

water boils at 100 degrees celsius which is insufficient to achieve sterilization.
although there is a method to sterilize using boiling water, its not the one above

dry heat sterilization is achieved at 160 degrees celsius for at least 2 hours which renders methods 2 and 4 useless (possibly more for oils)

also note for method 1 to work, the gear would need to be filtered directly into a sterile vial using a sterile filter housing and membrane and that BA is not used to achieve sterilization, but to preserve it
 
So other methods could work but with higher healing temperatures? Why are they so well speard?

What about microwave oven ?
 
Sorry guys, but I need to close this thread because it touches on a sensitive area that we can't allow.

All that I'll say in closing is that the posted article is full of a lot of bullshit.
 
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