I did some research on how to reconstitute TB500 and found:
1-0.9% Sodium chloride
2-BA water
3-0.1% AA
1 and 2 are by far the most common solvents advised. But I got 2 good papers that say AA is the way 2go. Both are big peptide producing companies.
What should I do; the advise on AAS boards is not the same as the factsheets?
A)
After reconstitution freezing @-20C never heard of freezing my peptides?
B)
So 0.1% AA is advised in both factsheets. But factsheet B says store @-20C but not after reconstitution (Am I right, English is not my first language)
Sources:
A) hoelzel-biotech.com/fileadmin/import/pdf/Aviva/OEMA00011__MANUAL.pdf
B)abcam.com/Thymosin-beta-4-peptide-1-4-ab42291.html
So what kind of solvent do I use and at what temperature do I keep the powder and the reconstituted stuff?
1-0.9% Sodium chloride
2-BA water
3-0.1% AA
1 and 2 are by far the most common solvents advised. But I got 2 good papers that say AA is the way 2go. Both are big peptide producing companies.
What should I do; the advise on AAS boards is not the same as the factsheets?
A)
Reconstitute in 0.1% acetic acid......Store lyophilized product at 2 to 8C. After reconstitution, store at -20C.
After reconstitution freezing @-20C never heard of freezing my peptides?
B)
Lyophilised:
Store at 2 to -8°C lyophilised, resuspend in 0.1% acetic acid....Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
So 0.1% AA is advised in both factsheets. But factsheet B says store @-20C but not after reconstitution (Am I right, English is not my first language)
Sources:
A) hoelzel-biotech.com/fileadmin/import/pdf/Aviva/OEMA00011__MANUAL.pdf
B)abcam.com/Thymosin-beta-4-peptide-1-4-ab42291.html
So what kind of solvent do I use and at what temperature do I keep the powder and the reconstituted stuff?