What the hell just happened??

[alexus hensicus]

Don't aspirate too hard too you don't want to crush your cell walls... just a flick with your finger nail

 

[Lightspeed81]

Really? Is that all it takes?? I usually pull a little on the plunger until I see a bubble!
How many of you after loading the syringe pull a small amount of air in before injecting? I was told to do this as it helps to scavenge the last of the gear out of the neck of the syringe & needle!!