you dont need it with this process..the test will precipitate and that is what you will filter out.. the estradiol will become soluble in water.. thats why rinsing is crucial
i can go into more detail at another time if you like or you can search a bit on bb.com mindandmuscle.net
sorry i just dont have the time now pm EMAIL me for more details if you need them and i can get back to you..
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