How many Iu are you injecting? Usually during cycle it's 250iu twice a week. It all depends on how much fluid you want to inject per. I like as little as possible. So per 5000iu vial, I put 5ml of BAC water in there. That makes 1000iu per 1ml. That means 250iu would be .25ml of quarter ml. per injectins. If you want more Bac in the injection, you can put 10ml of bac water which would be 250iu per .5ml of half ml