If you use NaOH in your experiment you will only make half of the estrogen soluble that is in the TP solution . Thats better than nothing as well you will strip the ester off the TP and will be left just test , that means you will have to inject you cow ED instead of EOD or you could just use D**ed's kit his estrogen solubilizer will make 99% of the estrogen soluble , and it will not strip the ester off . The NaOh will work just fine if you cannot afford D**ed's solubizer , just make sure to rinse the TP crystals with plenty of distilledwater and also make sure you check the ph level the way you do this is by using litmus paper , make sure that you touch the paper to the bottom of the coffee filter after you have rinsed the final product several times , i would recomend rinsing with at least a half of a gallon .
Good Luck