slindependant
New member
So i have been scouting around and came across this.......
Vitamin D’s Role in the Myostatin Pathway
1,25(OH)2Vitamin D3 Stimulates Myogenic Differentiation by Inhibiting Cell Proliferation and Modulating the Expression of Promyogenic Growth Factors and Myostatin in C2C12 Skeletal Muscle Cells
The study was relatively simple. The researchers split mouse C2C12 skeletal muscle cells into two groups: 1) a treated group (soaked in 100nM of vitamin D 2) an untreated group. Then over a 10 day period, the researchers used a handful of complex tests (immunefluorescence, PCR, Western blottings, ICC, etc) to answer two very important questions:
Does vitamin D autoregulate its own receptor?
Does vitamin D influence myogenesis?
The Vitamin D Receptor
Vitamin D does appear to autoregulate its owns receptor, which wasn’t that surprising. What was surprising was that it appeared to downregulate its receptor after several days of treatment, much like other hormones do.
Vitamin D and Muscle Growth
The results:
Up to an 85% reduction in PCNA expression (cell proliferation), P<0.0001
A 4-fold increase in MyoD expression, P<0.001
A 6-fold increase in desmin, P<0.001
A 2-fold increase in mean diameter of fibers, P<0.001
A 2.5-fold increase in size (width), P<0.001
A 3-fold decrease in IGF-1 expression, P<0.01
A A 2.5-fold increase in IGF-2 expression, P<0.01
Up to a 10-fold decrease in the expression of Myostatin, P<0.001
A 2.5-fold increase in Follistatin expression, P<0.01
Impressive, isn’t it? Not only did they have specific myogenic markers and transcription factors that suggest vitamin D’s role in muscular development, but they also had visible differences in fiber diameter and width.
One statistic was particularly interesting to me, and in my opinion, might be the most interesting finding in the study. Vitamin D appears to not only increase follistatin expression (which will bind any available myostatin and make it useless), but also appears to decrease myostatin production at the mRNA and protein level. So it can possibly reduce myostatin activity AND expression.
Does anyone here use or have tried vitamin D in such a way? i think i am going to try this but not sure whether to use it on cycle or off cycle to measure results also would anyone else be interested in taking part in doing this in the near future and reporting results to a thread?
Vitamin D’s Role in the Myostatin Pathway
1,25(OH)2Vitamin D3 Stimulates Myogenic Differentiation by Inhibiting Cell Proliferation and Modulating the Expression of Promyogenic Growth Factors and Myostatin in C2C12 Skeletal Muscle Cells
The study was relatively simple. The researchers split mouse C2C12 skeletal muscle cells into two groups: 1) a treated group (soaked in 100nM of vitamin D 2) an untreated group. Then over a 10 day period, the researchers used a handful of complex tests (immunefluorescence, PCR, Western blottings, ICC, etc) to answer two very important questions:
Does vitamin D autoregulate its own receptor?
Does vitamin D influence myogenesis?
The Vitamin D Receptor
Vitamin D does appear to autoregulate its owns receptor, which wasn’t that surprising. What was surprising was that it appeared to downregulate its receptor after several days of treatment, much like other hormones do.
Vitamin D and Muscle Growth
The results:
Up to an 85% reduction in PCNA expression (cell proliferation), P<0.0001
A 4-fold increase in MyoD expression, P<0.001
A 6-fold increase in desmin, P<0.001
A 2-fold increase in mean diameter of fibers, P<0.001
A 2.5-fold increase in size (width), P<0.001
A 3-fold decrease in IGF-1 expression, P<0.01
A A 2.5-fold increase in IGF-2 expression, P<0.01
Up to a 10-fold decrease in the expression of Myostatin, P<0.001
A 2.5-fold increase in Follistatin expression, P<0.01
Impressive, isn’t it? Not only did they have specific myogenic markers and transcription factors that suggest vitamin D’s role in muscular development, but they also had visible differences in fiber diameter and width.
One statistic was particularly interesting to me, and in my opinion, might be the most interesting finding in the study. Vitamin D appears to not only increase follistatin expression (which will bind any available myostatin and make it useless), but also appears to decrease myostatin production at the mRNA and protein level. So it can possibly reduce myostatin activity AND expression.
Does anyone here use or have tried vitamin D in such a way? i think i am going to try this but not sure whether to use it on cycle or off cycle to measure results also would anyone else be interested in taking part in doing this in the near future and reporting results to a thread?
