Steroids VS TRT?

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Different Lab Tests

Cool -- I've looked up both "PrivateMDLabs" and "IMT". I didn't really understand IMT ... not sure I found the right site. "Integrative Manual Therepy??" Is that correct?

PrivateMDLabs offered the testosterone tests below. I'm kind of a nerd and found it interesting reading, so I included it for people wondering more about testosterone testing. Also, the info below had some good overall info on testosterone and how it works in the body. I've starred (*) the ones I'm more interested in, and below I have some specific questions on those tests. Would love feedback:

* 1. Testosterone (Free) , Serum (Equilibrium Ultrafiltration) With Total Testosterone -- $88.49
Description: Testosterone levels decrease normally with age. This may cause men to have erectile problems or to lose interest in sex. This test will determine the level of free and total testosterone.

2. Testosterone, Free (Direct) With Total Testosterone, LC/MS-MS -- $89.49
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states in which testosterone-binding proteins may be altered (obesity, cirrhosis, thyroid disorders).

3. Testosterone, Free (Direct), Serum -- $68.49
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states where the testosterone binding proteins may be altered (obesity, cirrhosis, thyroid disorders)

4. Testosterone, Free (Direct), Serum With Total Testosterone -- $89.49
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states where the testosterone binding proteins may be altered (obesity, cirrhosis, thyroid disorders).

* 5. Testosterone, Free and Weakly Bound -- $89.49
escription: Free and weakly bound testosterone (FWBT), also referred to as bioavailable testosterone, is thought to reflect an individual's biologically active, circulating testosterone. FWBT includes free testosterone and testosterone that is bound to albumin. FWBT does not include sex hormone binding globulin-bound testosterone. The SHBG-bound fraction is biologically inactive because of the high binding affinity of SHBG for testosterone. The rapid dissociation of “weakly bound” testosterone from albumin results in the availability of essentially all albumin-bound testosterone for steroid-receptor interaction.

6. Testosterone, Free, Equilibrium Ultrafiltration With Total Testosterone, LC/MS-MS -- $198.99
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states in which the testosterone-binding proteins may be altered (obesity, cirrhosis, thyroid disorders).

7. Testosterone, Free, Mass Spectrometry/Equilibrium Dialysis -- $198.99
Description: Free testosterone is the fraction of total testosterone that is not bound to circulating proteins. The free fraction is available to diffuse into target cells to interact with receptors or enzymes. The concentration of free testosterone is low relative to total testosterone, typically <3% of the total testosterone concentration. Total circulating testosterone is bound to sex hormone-binding globulin (SHBG) or albumin. Measurement of total and free testosterone is key in diagnosis of androgen excess or deficiency. Free testosterone is best measured by equilibrium dialysis, while total testosterone is measured by HPLC with tandem mass spectrometry. Free testosterone is estimated in this profile using these recommended techniques. Tritiated testosterone is added to the sample and allowed to dialyze across a membrane. Once equilibrium has been achieved, the solutions on both sides of the membrane are sampled to determine the percent free testosterone. Total testosterone is measured by HPLC with tandem mass spectrometry. Serum free testosterone is then calculated by multiplying the percent free testosterone by the total testosterone concentration with unit correction.
This method is recommended for testing women, children, and hypogonadal men.

8. MoreTestosterone, Total - Women, Children, and Hypogonadal Males, LC/MS-MS -- $59.99
Description: This assay provides the sensitivity and specificity required for the assessment of the low testosterone levels found in women, children, adolescents, and hypogonadal men.

* 9. Testosterone, Total, Serum -- $44.49
Description: Testosterone testing is used to evaluate androgen excess or deficiency related to gonadal function, adrenal function, or tumor activity. Testosterone levels may be helpful in men for the diagnosis of hypogonadism, hypopituitarism, Klinefelter syndrome, and impotence (low values). Testosterone levels may be requested in women to investigate the cause of hirsutism, anovulation, amenorrhea, virilization, masculinizing tumors of the ovary, tumors of the adrenal cortices, and congenital adrenal hyperplasia (high values). Testosterone levels in children may be helpful to investigate issues related to puberty and development as well as the aforementioned. For testosterone measurements in females and children, use of Testosterone, Total, Women, Children, and Hypogonadal Males, LC/MS-MS, which employs liquid chromatography/tandem mass spectrometry (LC/MS-MS), is recommended

My questions:
1. Which test is most useful in determining testosterone levels before a steroid cycle in order to continuously track test levels before cycle, during cycle, after cycle, and after post cycle therapy (pct)? (1, 5, or 9?)
2. What is steroid-receptor interaction? How does test #5 connect with that?
3. What is the difference between "free" and "total" testosterone.
4. At what test levels should testosterone replacement therapy (TRT) be considered?
 
Cool -- I've looked up both "PrivateMDLabs" and "IMT". I didn't really understand IMT ... not sure I found the right site. "Integrative Manual Therepy??" Is that correct?

PrivateMDLabs offered the testosterone tests below. I'm kind of a nerd and found it interesting reading, so I included it for people wondering more about testosterone testing. Also, the info below had some good overall info on testosterone and how it works in the body. I've starred (*) the ones I'm more interested in, and below I have some specific questions on those tests. Would love feedback:

* 1. Testosterone (Free) , Serum (Equilibrium Ultrafiltration) With Total Testosterone -- $88.49
Description: Testosterone levels decrease normally with age. This may cause men to have erectile problems or to lose interest in sex. This test will determine the level of free and total testosterone.

2. Testosterone, Free (Direct) With Total Testosterone, LC/MS-MS -- $89.49
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states in which testosterone-binding proteins may be altered (obesity, cirrhosis, thyroid disorders).

3. Testosterone, Free (Direct), Serum -- $68.49
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states where the testosterone binding proteins may be altered (obesity, cirrhosis, thyroid disorders)

4. Testosterone, Free (Direct), Serum With Total Testosterone -- $89.49
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states where the testosterone binding proteins may be altered (obesity, cirrhosis, thyroid disorders).

* 5. Testosterone, Free and Weakly Bound -- $89.49
escription: Free and weakly bound testosterone (FWBT), also referred to as bioavailable testosterone, is thought to reflect an individual's biologically active, circulating testosterone. FWBT includes free testosterone and testosterone that is bound to albumin. FWBT does not include sex hormone binding globulin-bound testosterone. The SHBG-bound fraction is biologically inactive because of the high binding affinity of SHBG for testosterone. The rapid dissociation of ***8220;weakly bound***8221; testosterone from albumin results in the availability of essentially all albumin-bound testosterone for steroid-receptor interaction.

6. Testosterone, Free, Equilibrium Ultrafiltration With Total Testosterone, LC/MS-MS -- $198.99
Description: Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states in which the testosterone-binding proteins may be altered (obesity, cirrhosis, thyroid disorders).

7. Testosterone, Free, Mass Spectrometry/Equilibrium Dialysis -- $198.99
Description: Free testosterone is the fraction of total testosterone that is not bound to circulating proteins. The free fraction is available to diffuse into target cells to interact with receptors or enzymes. The concentration of free testosterone is low relative to total testosterone, typically <3% of the total testosterone concentration. Total circulating testosterone is bound to sex hormone-binding globulin (SHBG) or albumin. Measurement of total and free testosterone is key in diagnosis of androgen excess or deficiency. Free testosterone is best measured by equilibrium dialysis, while total testosterone is measured by HPLC with tandem mass spectrometry. Free testosterone is estimated in this profile using these recommended techniques. Tritiated testosterone is added to the sample and allowed to dialyze across a membrane. Once equilibrium has been achieved, the solutions on both sides of the membrane are sampled to determine the percent free testosterone. Total testosterone is measured by HPLC with tandem mass spectrometry. Serum free testosterone is then calculated by multiplying the percent free testosterone by the total testosterone concentration with unit correction.
This method is recommended for testing women, children, and hypogonadal men.

8. MoreTestosterone, Total - Women, Children, and Hypogonadal Males, LC/MS-MS -- $59.99
Description: This assay provides the sensitivity and specificity required for the assessment of the low testosterone levels found in women, children, adolescents, and hypogonadal men.

* 9. Testosterone, Total, Serum -- $44.49
Description: Testosterone testing is used to evaluate androgen excess or deficiency related to gonadal function, adrenal function, or tumor activity. Testosterone levels may be helpful in men for the diagnosis of hypogonadism, hypopituitarism, Klinefelter syndrome, and impotence (low values). Testosterone levels may be requested in women to investigate the cause of hirsutism, anovulation, amenorrhea, virilization, masculinizing tumors of the ovary, tumors of the adrenal cortices, and congenital adrenal hyperplasia (high values). Testosterone levels in children may be helpful to investigate issues related to puberty and development as well as the aforementioned. For testosterone measurements in females and children, use of Testosterone, Total, Women, Children, and Hypogonadal Males, LC/MS-MS, which employs liquid chromatography/tandem mass spectrometry (LC/MS-MS), is recommended

My questions:
1. Which test is most useful in determining testosterone levels before a steroid cycle in order to continuously track test levels before cycle, during cycle, after cycle, and after post cycle therapy (pct)? (1, 5, or 9?)
2. What is steroid-receptor interaction? How does test #5 connect with that?
3. What is the difference between "free" and "total" testosterone.
4. At what test levels should testosterone replacement therapy (TRT) be considered?



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