Yes oh yes, the day before your next injection is technically "your trough". But Doctors ask us to wait 7 days so there is a certain amount of decay, so the trough reflects a week of decay. A 7 day wait (decay) is the 'measuring stick', the 'standard' used by the medical community to measure our trough. The total TT in your body at time of injection is about the same, whether you inject daily, E3.5D or E7D, as most of the TT in your body is a build up of the last 8 weeks. But we need to use a 7 day wait to get a real trough IMHO; to not be at a disadvantage when measuring our TT; to use the same measuring standard that our Doctors use to evaluate us, not to be on a different page from our doctors.
Another example is: What if you were injecting every second day? Now your "trough" actually your Peak!! You would not really have a true trough. As far as E3.5D as a good protocol , I agree 1000% on that.
PS Halfwit: I appreciate your civil and professional tone used in this thread.
Ok, so what I think you're getting hung up on is trying to afford yourself the highest possible TT without being smacked on the hand by your doc because you went slightly out of the range they want to see you at.
That does make sense, yes. However, what really matters for how you feel and even look is the median, or average value you sit at over time. This is why I mentioned a steady state value, as that's the place where you're going to ultimately be spending most of your time anyway. Sure, you could even go with the original doctor preferred method of 400mg every 3-4 weeks, and feel GODLY for a few days, then feel like death as those hormones metabolize out your system. You'd have a total testosterone of around 2500ng+/dL for a while, but then crash down to around 200ng/dL.
Luckily, medical science is catching up to bro-science (sadly, the bb'ing community knew more for a long time in the mainstream) and they're catching on to how smaller doses do actually make things better for us if they're more frequently added. That's because if your hormones are stable, your body doesn't have to continually compensate.
So yes, if your doctor has you only an E7D protocol, it would be in your best interests to tell them that you would like to go with E3.5D as it will help smooth things out for you long-term. There are exceptions to this, but that falls into the realm outside TRT.
I personally feel and I have come to this conclusion like many others that what really matters in the grand scheme of things is how you feel when all is said and done. If your protocol has you at a peak of around 600ng/dL and you feel fantastic? More power to you. If you find that you require more, then as long as your doc is fine - go for it. That's the hard part of TRT; finding the right dose to fit your personal needs and lifestyle.
To answer the speculation about EOD, it's actually almost identical to E3.5D with respect to numbers as the doses are smaller. It really is only a big deal with much shorter esters that have half-lives in 2 day intervals.