? About goods left in media btl

web_dj

New member
Hello everyone! Is it safe to keep goods in the sterile media bottle for a few days after its filtered and capped? Or does anyone know of a way to transfer goods from the bottle to vials? I mean should I pour contents from the media bottle into a sterilized beaker and draw with clean pin and fill vials? Thanks for the input everyone I appreciate it.
 
Get yourself a silicone septa cap for your media bottle and sterile transfer with a large barrel into the smaller vials.
 
My thoughts too just didn't have one on hand so I was looking for alternative but your right I will do that you don't think I have to refilter it do you?
 
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It never hurts to refilter, but my thought of this is basically it will exposed to air during transfer.
The thing is, it will also be exposed to air with pressure change in the vial when you draw out oil and it sucks in a bit of air.
So as long as the solution was GTG and sterile originally, you should be ok.
Wouldn't hurt to keep all fans/wind off in room and spray a bit of lysol around prior to transfer either.
 
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