Do you think baking would kill anything the .45 let through that a .2 wouldn't? I'm just paranoid about not having the "right" filter.
Also I made a slight mistake. I forgot to set aside 2ml to clear the filter. Should I just go ahead and run another 2ml and just have a bit lower of a concentration
Don't compromise on your sterile technique. You'll see post on the internet amounting to "You're going to be filtering in the end anyway". Heating, Ultrasonic Cleaning, Autoclaving, Sterile Pipetting..... These are all part of process with the overall goal of maximum sterility for products intended for Intra-Muscular Injection in Humans.
A common mistake I see in forums, are people washing their glassware or vials with tap-water. This will void the .2 micron filter you use. You're going to end up with an abundance of particles above 2 microns. The reality is that even distilled water won't meet the specs required by the pharmacy industry. Stay clean