Whey protien stability

nekrawulf

New member
One of my co-workers kinda has me freaked out! He told me that allowing wey protien to sit for an extended period of time (2+ hours) reders it useless. I fix my protien shakes for the day early in the morning because it makes it easier for me...is that the wrong thing to do...does time really wreak havok on my whey?!
 
correct me if im wrong but i do believe that protein powders do get downgraded after awhile from sitting in any kinda liquid. im not sure what the deal is with RTD's. maybe someone can shed some light better then i can.
 
When you cook your chicken or steak, it does get dergaded a bit, but it just breaks down the protein for easier digestion, you still get all the amino acids, cals, etc.
 
proteins may be broken if exposed to light or higher temperatures, but what's the deal ?

the amino acids are still intact. that's what you need. you don't ingest proteins, they are broken in aa by enzymes in your lower stomach and intestine before being absorbed.

If you keep it cool, it should be just fine. Anyway, I'll study this, I have a biochemistry exam on may 2nd. If I find anything in my books, I'll tell you ;)
 
bassmanjp said:
proteins may be broken if exposed to light or higher temperatures, but what's the deal ?

the amino acids are still intact. that's what you need. you don't ingest proteins, they are broken in aa by enzymes in your lower stomach and intestine before being absorbed.

If you keep it cool, it should be just fine. Anyway, I'll study this, I have a biochemistry exam on may 2nd. If I find anything in my books, I'll tell you ;)

Yes.....but the question is : Once mixed with water, does it start to break down to the point where it is no good.
 
i cant see this being true,,, i mix a shack all the time i go to work and it sits for like 5-6 hours in the fridge, i still gained alota weight recently, but yea if someone can really find ome hard evidence then that would be cool.
 
StoneColdNTO said:
Yes.....but the question is : Once mixed with water, does it start to break down to the point where it is no good.

I wouldn't think so.

To say that it is no good, your AA have to be altered. And it won't be altered in water.

Some of the proteins might break down in AA, but that's as far as it goes.

Except if you really boil hard your water, you mix with some strong acid or strong base, or any hard chemical treatments, your AA won't break down.

The bonds within each AA molecules are quite strong and it is a hard reaction to break those bonds without enzymes that are found in human body (and other animals, some bacterias, etc).

The only alteration that might happend to your AA is that they will be ionized, but it doesn't matter at all, because you stomach contains HCl, which is a strong acid, and your intestine has some bicarbonate to neutralize the acid... so your AA will go through all kind of ionization forms anyway.
 
RoadHouse said:
When you cook your chicken or steak, it does get dergaded a bit, but it just breaks down the protein for easier digestion, you still get all the amino acids, cals, etc.



This is exactly right.
The word is "denatured" actually, which refers to the deformation of the protein's unique 3-dimensional shape, usually by heat or chemical influence. Not sure how that would happen in water, but water is a chemical, too, so I imagine it may occur. It would occur very slowly though.
As has been said, there's no change in the amino acid complement of the stuff.
 
buffdoc said:
This is exactly right.
The word is "denatured" actually, which refers to the deformation of the protein's unique 3-dimensional shape, usually by heat or chemical influence. Not sure how that would happen in water, but water is a chemical, too, so I imagine it may occur. It would occur very slowly though.
As has been said, there's no change in the amino acid complement of the stuff.

Exact too bro.

As a final answer to this post, i'll let you know this:

when we want to know of which AA a protein is made of, we have to break the protein into single AA. to do this, we hydrolyze the protein with hydrochloridric acid (HCl 6N) at 110 degrees Celcius for 24 hours... and this doesn't alter the AA.

So, for your protein shake to stay in water which pH is about 7, temperature between 2 and 20 degrees, for a few hours, it won't do a thing, probably not to your proteins and surely not to your AA.

I hope this is helping to make things clear. If something's not clear in some of my posts, let me know. I'm doing my best with English language :)
 
bassmanjp said:
Exact too bro.

As a final answer to this post...................

Well, I'm still not convinced.

How long have ready to drink protein drinks been around for....only a few years.
How long has there been whey protein powder.....a lot longer...right ?

There has to be something to it. It's not like one day, someone just said, "Hey, why don't I mix up some protein powder in water, bottle it and make millions of dollars."

See where I'm going with this ??
 
StoneColdNTO, i clearly understand your point.

Only, something that I'm pretty sure of is that if you leave your drink overnight in the fridge, I will be good tomorrow.

As the question about the stability over a longer period of time, then it can be different.

You dried protein powed will have a great advantage of aqueous protein solutions. The fact that it is dried will prevent any bacterial growth.

But this can be easely solved for liquid protein solutions if you take care of the sterility. However, it is easy to see that this would be hard to do for you at home, make sure everything is bacteria-free.

If you want to look over stability over a long period of time, proteins should react the same way as drugs (many of them are proteins). Different factors can affect the stability of your protein solution, such as temperature, light, humidity, oxygen or carbone dioxyde presence. Still, this is not a big problem for liquid protein solutions. You make an opaque sealed container and you try to keep it cool. (I don't know much about liquid protein solutions handling, does the manufacturer suggest a temperature at which the solution should be kept ?)

Even though, theses factors can be controlled. But, what I think might happen to these solutions over an extended period of time, is that the amino acids orientation changes.

Your AA can be either L or R, just as L-Glutamine or R-Glutamine. The human body can only use L-AA. Because water is a polar solvant, thus it can allows ions exchange, your AA might over a long period of time changes from L to R, which might diminish the available quantity of protein in your drink.

That's the only thing I see that might happen.
 
Hey bassmanjp........

Nice reply....you sure seem to know your stuff.

In a previous post you said, "I hope this is helping to make things clear. If something's not clear in some of my posts, let me know. I'm doing my best with English language"

Well I gotta say, you have a very good command of the English language.

If you don't mind me asking, where are you from and what are you studying ?
 
StoneColdNTO said:
Hey bassmanjp........

Nice reply....you sure seem to know your stuff.

In a previous post you said, "I hope this is helping to make things clear. If something's not clear in some of my posts, let me know. I'm doing my best with English language"

Well I gotta say, you have a very good command of the English language.

If you don't mind me asking, where are you from and what are you studying ?

I live in Montreal and studying to be a pharmacist :)

or who knows.. i'll get through my 4-year program then i'll see where i'll go... ! :)
 
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