Heart Health, Liver Health, Anti-Depressant/Mood Elevator.....TMG is the stuff!


I am banned!
TMG (Trimethylglycine)

Potent Methyl-donor:

Methylation is a natural process of transfer of methyl groups (-CH3) between different compounds. This can serve to activate, recycle, detoxify and/or protect the affected molecules. Research shows that decreased methylation is involved in many health conditions, including diseases of the cardiovascular system, brain and liver, as well as cancer. Methylation is a key biochemical step ocurring a billion times a second. A coating of methyl groups on DNA protects against aging, oxidation and genetic damage.

Trimethylglycine (TMG) is an excellent source of methyl groups and is found in good quantities in broccoli and beets. TMG (Trimethyl glycine) is also known as anhydrous betaine, glycine betaine, or oxyneurine. It is a white crystalline powder with a naturally sweet taste and it disolves readily under the tongue for sublingual absorption, or for those who prefer, in water or other drinks. TMG is sometimes confused with betaine hydrochloride, which is also found in nutritional products, but as a stomach acidifier.


The focus on dietary cholesterol has thrown many people off the track. Dietary cholesterol is not the main source of blood cholesterol. And blood cholesterol is not the main risk factor for cardiovascular disease. Animals fed normal diets with the addition of high amounts of cholesterol do not develop atherosclerosis. A much higher risk factor is elevated homocysteine (HCY) levels. In the Physicians(1) Health Study, doctors with high homocysteine levels were found to have 3 to 4 times higher risk for heart attack compared to those with lower levels.

Four major modifiable factors affect homocysteine levels:

The rate of conversion of homocysteine to methionine via methylation
the rate of homocysteine conversion to cystathionine and cysteine via transsulfuration the amount of homocysteine excreted into the plasma by cells the level of methionine in the diet.

TMG is one of the most powerful methylating agents known and has been shown to lower homocysteine levels when nothing else would.


Methylation regulates the expression of genes by coating the DNA, selectively turning some genes on and others off. Young people typically start out with enough methyl groups to regulate genetic activity, but these are lost during mitosis (cell division) due to dietary deficiency. The result is expression of oncogenes, which leads to cancer. Optimum methylation of the DNA prevents cancer by inhibiting oncogenes. Dietary intake of high levels of methyl donors is linked to low cancer rates, and animal studies show that methyl donor supplementation prevents cancer in .


DNA methylation also helps explain the mechanisms of aging. DNA expression determines the building blocks and functions of all cells. With aging-related loss of DNA methylation, genetic expression changes. Methylation is a process of biochemical reduction (addition of electrons), the opposite of oxidation (loss of electrons). TMG is a potent antioxidant as well as a methyl donor, so it feeds both sides of the age-reversal equation. TMG also helps oxygenation at the cellular level, which further reduces aging related oxidation of the cell structures such as DNA and cell membrane. Oxygen is needed as a receptor for spent electrons from mitochondrial respiration, to prevent backup of electrons in the electron transport chain leading to leakage of high energy electrons (free radicals). By reversing atherosclerosis, TMG also provides enhanced circulation and tissue oxygenation over time with supplementation.

Mood-Elevation and Liver-Health:

Methylation increases production of S-adenosyl-methionine, or SAMe. SAMe is an excellent antidepressant without the side effects of trycyclics. SAMe is very useful in liver disease and in osteoarthritis it has been shown to stimulate cartilage regeneration. TMG is an excellent way to elevate bodily SAMe synthesis, and should always be taken by those supplementing SAMe according to the Remission Foundation. When SAMe is spent, it loses a methyl group to become homocysteine. TMG recycles the homocysteine back into SAMe, so with enough TMG in the system, the same SAMe molecule can be used over and over, never staying in the toxic homocysteine form for long enough to damage the vascular system.


Evaluation of radiation injury by 1H and 31P NMR of human urine.

Yushmanov VE

Institute of Chemical Physics, Russian Academy of Sciences, Moscow.

Magn Reson Med, 1994 Jan, 31:1, 48-52

1H and 31P NMR techniques were applied to study the changes in metabolite profiles in human urine resulting from radiation exposure following the Chernobyl reactor accident. In cases of acute leukemia and different accumulated doses of external radiation (from 0.20 to 4.00 Sv), the proton spectra were classified on the basis of the peaks due to N-trimethyl groups, creatinine, citrate, glycine, and hippurate. Unidentified resonances were observed between 15.9 and 21.4 ppm in six phosphorus spectra of patients with preleukemia and acute leukemia. Characteristic spectral changes were similar for external radiation and incorporation-induced internal irradiation. The spectral patterns described may serve as a criterion of radiation injury.

Unique Identifier 94166611


Betaine:homocysteine methyltransferase--a new assay for the liver enzyme and its absence from human skin fibroblasts and peripheral blood lymphocytes.

Wang JA; Dudman NP; Lynch J; Wilcken DE

Department of Cardiovascular Medicine, Prince Henry Hospital, University of New South Wales, Sydney, Australia.

Clin Chim Acta, 1991 Dec 31, 204:1-3, 239-49

Chronic elevation of plasma homocysteine is associated with increased atherogenesis and thrombosis, and can be lowered by betaine (N,N,N-trimethylglycine) treatment which is thought to stimulate activity of the enzyme betaine:homocysteine methyltransferase. We have developed a new assay for this enzyme, in which the products of the enzyme-catalysed reaction between betaine and homocysteine are oxidised by performic acid before being separated and quantified by amino acid analysis. This assay confirmed that human liver contains abundant betaine:homocysteine methyltransferase (33.4 nmol/h/mg protein at 37 degrees C, pH 7.4). Chicken and lamb livers also contain the enzyme, with respective activities of 50.4 and 6.2 nmol/h/mg protein. However, phytohaemagglutinin-stimulated human peripheral blood lymphocytes and cultured human skin fibroblasts contained no detectable betaine:homocysteine methyltransferase (less than 1.4 nmol/h/mg protein), even after cells were pre-cultured in media designed to stimulate production of the enzyme. The results emphasize the importance of the liver in mediating the lowering of elevated circulating homocysteine by betaine.

Unique Identifier 92306342


Country of Publication NETHERLANDS

CAS Registry/EC Number EC 2.1.1. (Methyltransferases); EC (betaine-homocysteine methyltransferase); 107-43-7 (Betaine); 454-28-4 (Homocysteine); 7005-18-7 (Methionine)

1H NMR determination of urinary betaine in patients with premature vascular disease and mild homocysteinemia.

Lundberg P; Dudman NP; Kuchel PW; Wilcken DE Address Department of Biochemistry, University of Sydney, NSW, Australia.

Clin Chem, 1995 Feb, 41:2, 275-83

Urinary N,N,N-trimethylglycine (betaine) and N,N-dimethylglycine (DMG) have been identified and quantified for clinical purposes by proton nuclear magnetic resonance (1H NMR) measurement in previous studies. We have assessed these procedures by using both one-dimensional (1-D) and 2-D NMR spectroscopy, together with pH titration of urinary extracts to help assign 1H NMR spectral peaks. The betaine calibration curve linearity was excellent (r = 0.997, P = 0.0001) over the concentration range 0.2-1.2 mmol/L, and CVs for replicate betaine analyses ranged from 7% (n = 10) at the lowest concentration to 1% (n = 9) at the highest. The detection limit for betaine was < 15 mumol/L. Urinary DMG concentrations were substantially lower than those of betaine. Urinary betaine and DMG concentrations measured by 1H NMR spectroscopy from 13 patients with premature vascular disease and 17 normal controls provided clinically pertinent data. We conclude that 1H NMR provides unique advantages as a research tool for determination of urinary betaine and DMG concentrations.

Unique Identifier 95179851


:COPY-CLAIM: 2001: G. M. Swartwout

A little more on the stuff..... ;)

Another information sheet from Kirkman Labs provides more information on TMG. It stated that Trimethyglycine (TMG) DMG’s big brother that does so much more!

How is TMG Different from DMG?

In simple terms TMG is DMG with one more methyl group. TMG stands for Trimethyglycine while DMG stands for Dimethyglycine.

Dimethyglycine (DMG) is well known in the autistic community. …Not many people however know about its “Big Brother”, Trimethyglycine (TMG). In short, TMG provides several remarkable benefits and then becomes DMG.

TMG donates one methyl group (it has 3 while DMG has 2) that reduces harmful homocysteins and increases beneficial seratonin by stimulating the production of a precursor called SAMe. The remaining two methyl are DMG!!! The formula is 250 mg of TMG will result in 125 mg of DMG.

What is Trimethyglycine (TMG)?

TMG is a naturally found chemical compound found in some foods. It can be extracted from sugar beets in a very concentrated form. The result is a 99% pure white crystallized powder with a distinctive taste. It is mildly sweet with a mild aftertaste.

TMG is also known as glycine betaine.

What is the Benefit of TMG?

TMG is the newest and most effective methylation-enhancing compound. Methylation is a natural process that makes new cells, creates natural antioxidants and breaks down harmful chemicals.

TMG converts one of these harmful chemicals, toxic homocysteine into methionine. It also boosts the level of beneficial SAMe and then becomes DMG (Dimethyglycine). It takes approximately 250 mg of TMG to naturally produce 125 mg of DMG.

This methylation of TMG produces the following benefits:

1. TMG methylation lowers dangerous homocysteine levels, thus lowering the risk of heart disease and stroke. Methylation is the primary process by which homocysteine is transformed into nontoxic compounds such as SAMe.

2. TMG methylation produces SAMe, which may have potent anti-aging effects, and has been shown to alleviate depression, remylenate nerve cells, improve Alzheimer’s and Parkinson’s disease patients, and protect against alcohol-induced liver injury.

3. Methylation protects DNA damage.

I get mine at Beyond-a-century.com in dirt cheap tasteless powder form!!
TMG (Betaine Anhydrous) is a methyl donor with the following applications in human nutrition:

TMG helps metabolize fats, which allows the body to burn fat rather than protein or muscle during exercise. The result is less cramping, increased endurance, and better utilization of fat stores

Dose range: 1,000-2,000 mg daily.

Anti-depression: By raising level of beneficial SAM (S-Adenosyl-Methionine).

Dose range: 500-1,500 mg daily

Body building: In the animal husbandry field, TMG is used to decrease fat and increase meat yield. While human studies have just started, a 200 pound individual with 20% body fat can expect to lose as much as 5 pounds of fat and gain as much as 12 pounds of muscle.

Dose range: 500-2,000 mg daily

Cardiac protection: Conversion of homocysteine to Methionine.

Dose range: 500-1,000 mg daily

General Health Preventative: As part of a formula for maintaining good health or disease prevention.

Dose range: 500-1,000 mg daily

Glutathione Elevation:In several different studies, TMG has been shown to increase hepatic Glutathione, the body's most important antioxidant.

Dose range: 500-1,500 mg daily

Homocysteine Lowering:Specific for that purpose. The supplement of choice for lowering homocystenuria.

Dose range: 500-1,000 mg daily.

As used by clinicians and hospitals with patient monitoring -up to 3 grams daily

Liver Disorders: As a part of liver-healing and protection formulas. Increases SAM levels in the liver, enables the liver to metabolize fat and protect against many challenges such as alcohol induced cirrhosis. TMG will also decrease bilirubin, alkaline phosphotase, and several other liver enzymes related to a large variety of liver disorders. Significant liver benefits have been shown in 20 studies.

Dose range: 500-1,500 mg. daily.

Longevity Formulations: As a part of a life extension formula. TMG has shown ability to protect interrogate of cellular DNA through methyl donation.

Dose range: 500-1,000 mg daily.

Methyl Donor Formulations: Along with B12, Folic acid, and Choline.

Dose range: 500-1,500 mg. daily.

Performance: Due to it's bi-polar nature, helps osmotic pressure in cells. For example it is used in salmon farming to protect fish against the problems of changing salt content. In humans, TMG maintains normal cellular electrolyte concentrations despite water and electrolyte losses during exercise.
house1 said:
doc, where can you get this?????

Originally posted by DRveejay11
I get mine at Beyond-a-century.com in dirt cheap tasteless powder form!!



""TMG, TriMethylGlycine, aka betaine base, a natural metabolic product of choline, is a methyl donor which lowers levels of plaque-forming homocysteine and raises levels of SAMe, a powerful antidepressant and anti-arthritis substance. In the liver, TMG helps metabolize fat, and increases detoxifying liver enzymes and antioxidant glutathione levels. After giving up a methyl group, it turns into antiaging sports nutrient *533 DMG. In farm animals, TMG is used to decrease fat and increase meat production. Suggested daily dose is 500-1500mg with other anti-homocysteine nutrients: B6, B12, folic acid, & choline. People with depression or liver disease take up to 6 grams a day safely. Sweet taste in water. 150 grams, $6.75. Code 034.0""
thanks brother -- just ordered some--been looking for something like this
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Great thread DrV.

I decided to try TMG based on some posts you and Nelson M. put up some time ago in a thread that was started about bromocriptine. I bought a bunch of anti-oxidant / life extension powders from Beyond-A-Century and I made big caps, which I take 3x per day. Here is the contents of each cap:

300 mg. TMG
200 mg. R-ALA
200 mg. Biotin powder (it is diluted)
200 mg. NAC
100 mg. carnosine
100 mg. silymarin
Trevdog said:
Great thread DrV.

I decided to try TMG based on some posts you and Nelson M. put up some time ago in a thread that was started about bromocriptine. I bought a bunch of anti-oxidant / life extension powders from Beyond-A-Century and I made big caps, which I take 3x per day. Here is the contents of each cap:

300 mg. TMG
200 mg. R-ALA
200 mg. Biotin powder (it is diluted)
200 mg. NAC
100 mg. carnosine
100 mg. silymarin

DAMN!! Talk about a healthy-ass supp regimine ;)


Now cap me some :D
about how long til you feel the effects?

I just ordered some.. Im mostly interested in the cardio vascular and joint benefits.